Loading...
This record has been deleted. Since it is linked to a published record, it is available here for reference purposes only.
Click here to view the deleted recordThis document has been updated. This is not the latest published version. Click here to view the latest version of the record.
Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Cowpea resistant to maruca pod borer
EN
AAT709A × 245F
Yes
AAT-7Ø9AA-4 × AAT-Ø245F-3
-
Person:AATFP.O. Box 30709Nairobi,
00100, KenyaPhone: + 254-20 422 3700,Fax:Email: aatf@aatf-africa.org,Website: http://www.aatf-africa.org/en/,Related OrganizationThe African Agricultural Technology Foundation (AATF) ()Non-governmental organization (NGO)P.O. Box 30709Nairobi,
00100, KenyaPhone: + 254-20 422 3700,Fax:Email: aatf@aatf-africa.org,Website: http://www.aatf-africa.org/en/,
Cowpea (Vigna unguiculata) was modified through cross breeding of two parental lines for resistance to Lepidoptera pests, particularly Maruca vitrata (pod borer). The modified cowpea expresses Bacillus thuringiensis crystal (Bt) proteins Cry1Ab and Cry2Ab, which have a pore-forming mode of action that selectively damages the midgut epithelium line of feeding larvae, resulting in death of the feeding larvae. The parental lines were crossed to include the Cry2Ab cassette in addition to the Cry1Ab to address the potential formation of resistance in pests. In addition to the Bt gene cassettes, the modified cowpea expresses two Escherichia coli neomycin phosphotransferase II cassettes, which functioned as a selectable marker for kanamycin resistance during transformation of the parental lines.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
-
BCH-ORGA-SCBD-103617-3 Organism Vigna unguiculata (Cowpea, Black eyed pea)Crops
-
BCH-LMO-SCBD-114444-2 Living Modified Organism AAT-7Ø9AA-4 - Pod Borer-resistant cowpeaAfrican Agricultural Technology Foundation and Institute of Agricultural Research | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
-
BCH-LMO-SCBD-269931-1 Living Modified Organism AAT-Ø245F-3 - Pod borer-resistant cowpeaAfrican Agricultural Technology Foundation and Institute of Agricultural Research | Resistance to antibiotics (Kanamycin, Neomycin), Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), Cotton bollworm (Helicoverpa spp.), European corn borer (Ostrinia nubilalis), Fall armyworm (Spodoptera frugiperda)), Selectable marker genes and reporter genes
EN
-
Cowpea resistant to lepidoptera pests| The African Agricultural Technology Foundation (AATF) | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), European corn borer (Ostrinia nubilalis))
pMB4; pMB6
EN
- Cross breeding
|
0.523 kb
|
|
0.177 kb
|
|
0.190 kb
|
|
0.618 kb
|
|
0.139 kb
|
|
1.726 kb
|
|
0.273 kb
|
|
1.905 kb
|
|
0.373 kb
|
|
0.523 kb
|
|
0.117 kb
|
|
0.119 kb
|
|
0.618 kb
|
|
0.139 kb
|
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
-
BCH-GENE-SCBD-114438-3 SCSV S1 promoter | Subterranean clover stunt virus (SCSV, Subterranean clover stunt virus)Promoter
-
BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
-
BCH-GENE-SCBD-114440-2 SCSV3 terminator | Subterranean clover stunt virus (SCSV, Subterranean clover stunt virus)Terminator
-
BCH-GENE-SCBD-103851-5 rbcS Promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Promoter
-
BCH-GENE-SCBD-14988-7 Cry2Ab2 | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
-
BCH-GENE-SCBD-103853-2 rbcS Terminator | Nicotiana tabacum (Tobacco, TOBAC )Terminator
-
BCH-GENE-SCBD-14985-12 Cry1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
-
BCH-GENE-SCBD-114274-2 Catalase 1 intron | Ricinus communis (Castor bean)Intron
-
BCH-GENE-SCBD-101902-5 Ribulose-1,5-bisphosphate carboxylase small subunit transit peptide | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Transit signal
DNA insert from AAT709A (AAT-7Ø9AA-4) vector pMB4:
The parental line contains two gene cassettes: Bacillus thuringiensis delta-endotoxin (crystal) cry1Ab and Escherichia coli neomycin phosphotransferase II (nptII).
Transcription of cry1Ab occurs from the Arabidopsis thaliana ribulose-1,5-bisphosphate carboxylase small subunit promoter and terminates at the Nicotiana tabacum ribulose-1,5-bisphosphate carboxylase small subunit terminator.
Transcription of nptII is controlled by a Subterranean clover stunt virus DNA segment 1 promoter and DNA segment 3 terminator. The coding sequence of nptII is interrupted by the presence of a Ricinus communis catalase 1 intron to prevent leaky expression of the nptII gene cassette. Due to the nature of the promoter, elevated levels of transcription of nptII are expected from this gene cassette.
Note:
The parental line contains two gene cassettes: Bacillus thuringiensis delta-endotoxin (crystal) cry1Ab and Escherichia coli neomycin phosphotransferase II (nptII).
Transcription of cry1Ab occurs from the Arabidopsis thaliana ribulose-1,5-bisphosphate carboxylase small subunit promoter and terminates at the Nicotiana tabacum ribulose-1,5-bisphosphate carboxylase small subunit terminator.
Transcription of nptII is controlled by a Subterranean clover stunt virus DNA segment 1 promoter and DNA segment 3 terminator. The coding sequence of nptII is interrupted by the presence of a Ricinus communis catalase 1 intron to prevent leaky expression of the nptII gene cassette. Due to the nature of the promoter, elevated levels of transcription of nptII are expected from this gene cassette.
Note:
- The coding sequence of cry1Ab was codon optimized for plant expression.
- Southern blot analysis identified a single T-DNA insertion in the genome, comprising of one complete copy of the plasmid pMB4 T-DNA, except for 10 bp and 29 bp truncations at the 5’ and 3’ termini, respectively, and a 735 bp inverted repeat derived from the 3’ end of the T-DNA. Further, the site of insertion of the T-DNA was mapped to a region on chromosome 2 of the cowpea genome.
DNA insert from 245F (AAT-Ø245F-3) vector pMB6:
The parental line contains two gene cassettes: Bacillus thuringiensis cry2Ab2 and Escherichia coli neomycin phosphotransferase II (nptII).
Transcription of cry2ab2 is controlled by an Arabidopsis thaliana ribulose-1,5-bisphosphate carboxylase small subunit promoter and Nicotiana tabacum ribulose-1,5-bisphosphate carboxylase small subunit terminator. The transit peptide of the ribulose-1,5-bisphosphate carboxylase small subunit coding sequence has also been included to direct the translated peptide to the chloroplasts.
Transcription of nptII is controlled by a Subterranean clover stunt virus DNA segment 1 promoter and DNA segment 3 terminator. The coding sequence of nptII is interrupted by the presence of a Ricinus communis catalase 1 intron to prevent leaky expression of the nptII gene cassette. Due to the nature of the promoter, elevated levels of transcription of nptII are expected from this gene cassette.
Note:
- Sequencing analysis indicated that a single insertion of the T-DNA was present in the parental genome and 41-basepair and 26-basepair truncations at the 5’ and 3’ termini, respectively, occurred. Further, analysis of 5’ and 3’ flanking host genomic DNA, the site of T-DNA insertion was mapped to an intergenic region on chromosome 1, and there were no new novel junction-spanning open reading frames created as a consequence of the genetic transformation.
For more information, kindly refer to the parental LMO records.
EN
- Food
EN
EN
| Record type | Field | Record(s) | |
|---|---|---|---|
| Country's Decision or any other Communication | LMO identification | 2 | |
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 2 | |
